Avicenna Journal of Clinical Microbiology and Infection

Published by: Kowsar

Antibacterial Activity of Azadirachta indica Leaf Extracts Against Some Pathogenic Standards and Clinical Bacterial Isolates

Leila Maleki 1 , Tahereh Sadeghian-Rizi 2 , * , Mostafa Ghannadian 3 , Mohammad Hossein Sanati 4 , Shahin Shafizadegan 5 and Hojjat Sadeghi-Aliabadi 6 , **
Authors Information
1 M.Sc. of Biotechnology, Department of Microbial Biotechnology, School of Biology Sciences, Nour Danesh Institute of Higher Education, Meymeh, Isfahan, Iran
2 Ph.D. Candidate in Pharmaceutical Biotechnology, Department of Pharmaceutical Biotechnology, School of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran
3 Ph.D., Department of Pharmacognosy, School of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran
4 Ph.D., Department of Medical Genetics, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran
5 B.SC of Biology, Department of Pharmaceutical Biotechnology, School of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran
6 Ph.D., Department of Pharmaceutical Biotechnology, School of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran
Corresponding Authors:
Article information
  • Avicenna Journal of Clinical Microbiology and Infection: February 2018, 5 (1); e12987
  • Published Online: November 27, 2017
  • Article Type: Research Article
  • Received: May 10, 2017
  • Revised: October 6, 2017
  • Accepted: November 22, 2017
  • DOI: 10.5812/ajcmi.12987

To Cite: Maleki L, Sadeghian-Rizi T, Ghannadian M, Sanati M H, Shafizadegan S, et al. Antibacterial Activity of Azadirachta indica Leaf Extracts Against Some Pathogenic Standards and Clinical Bacterial Isolates, Avicenna J Clin Microb Infec. 2018 ;5(1):e12987. doi: 10.5812/ajcmi.12987.

Copyright © 2017, Avicenna Journal of Clinical Microbiology and Infection. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited
1. Background
2. Objectives
3. Methods
4. Results
5. Discussion
  • 1. Packer J, Naz T, Yaegl Community E, Harrington D, Jamie JF, Vemulpad SR. Antimicrobial activity of customary medicinal plants of the Yaegl Aboriginal community of northern New South Wales, Australia: a preliminary study. BMC Res Notes. 2015;8:276. doi: 10.1186/s13104-015-1258-x. [PubMed: 26122212].
  • 2. Sarker SD, Nahar L, Kumarasamy Y. Microtitre plate-based antibacterial assay incorporating resazurin as an indicator of cell growth, and its application in the in vitro antibacterial screening of phytochemicals. Methods. 2007;42(4):321-4. doi: 10.1016/j.ymeth.2007.01.006. [PubMed: 17560319].
  • 3. Chanda S, Nair R. Activity of some medicinal plants against certain pathogenic bacterial strains. Indian J Pharmacol. 2006;38(2):142-4. doi: 10.4103/0253-7613.24625.
  • 4. Ahmed MF, Rao AS. Simultaneous determination of phenolic compounds in melia azedarach. Linn leaves by high-performance liquid chromatography. Indian J Appl Res. 2011;3(11):429-31. doi: 10.15373/2249555x/nov2013/137.
  • 5. Jafari S, Saeidnia S, Hajimehdipoor H, Ardekani MR, Faramarzi MA, Hadjiakhoondi A, et al. Cytotoxic evaluation of Melia azedarach in comparison with, Azadirachta indica and its phytochemical investigation. Daru. 2013;21(1):37. doi: 10.1186/2008-2231-21-37. [PubMed: 23679992].
  • 6. Zargari A. Treatment with plants, Pharmacogenosis [In Persian]. Tehran: Tehran University Publications; 1990. 67 p.
  • 7. Sharma C, Vas AJ, Goala P, Gheewala TM, Rizvi TA, Hussain A. Ethanolic Neem (Azadirachta indica) Leaf Extract Prevents Growth of MCF-7 and HeLa Cells and Potentiates the Therapeutic Index of Cisplatin. J Oncol. 2014;2014:321754. doi: 10.1155/2014/321754. [PubMed: 24624140].
  • 8. Banna QR, Parveen F, Iqbal J. Growth inhibitory effect of ethanolic neem leaves extract on Klebsiella, Salmonella and Staphylococcus aureus. Bangladesh J Pharmacol. 2014;9(3):347-50. doi: 10.3329/bjp.v9i3.19454.
  • 9. Ferraro MJ. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically: approved standard. Ninth ed. Wayne, PA: NCCLS; 2012.
  • 10. Murray PR, Baron EJ, Pfaller MA, Tenover FC, Yolken HR. Manual of clinical microbiology. 6th ed. Washington DC: ASM Press; 1995. p. 15-8.
  • 11. Steenkamp V, Mathivha E, Gouws MC, van Rensburg CE. Studies on antibacterial, antioxidant and fibroblast growth stimulation of wound healing remedies from South Africa. J Ethnopharmacol. 2004;95(2-3):353-7. doi: 10.1016/j.jep.2004.08.020. [PubMed: 15507359].
  • 12. da Costa LS, Andreazza NL, Correa WR, Cunha IS, Ruiz ALTG, de Carvalho JE, et al. Antiproliferative activity, antioxidant capacity and chemical composition of extracts from the leaves and stem of Chresta sphaerocephala. Rev Brasil Farmacog. 2015;25(4):369-74. doi: 10.1016/j.bjp.2015.04.005.
  • 13. Kone WM, Atindehou KK, Terreaux C, Hostettmann K, Traore D, Dosso M. Traditional medicine in north Cote-d'Ivoire: screening of 50 medicinal plants for antibacterial activity. J Ethnopharmacol. 2004;93(1):43-9. doi: 10.1016/j.jep.2004.03.006. [PubMed: 15182903].
  • 14. Malekzadeh F, Ehsanifar H, Shahamat M, Levin M, Colwell RR. Antibacterial activity of black myrobalan (Terminalia chebula Retz) against Helicobacter pylori. Int J Antimicrob Agents. 2001;18(1):85-8. doi: 10.1016/s0924-8579(01)00352-1.
  • 15. Reardon S. Antibiotic resistance sweeping developing world. Nature. 2014;509(7499):141-2. doi: 10.1038/509141a. [PubMed: 24805322].
  • 16. Uttamkumar B, Bhubaneswari A, Tejasri MVV. Comparative antibacterial activities of the combined crude leaf extract of bixa orellana, azadirachta indica and ocimum scantum. Int Res J Pharm. 2016;4(4):189-93. doi: 10.7897/2230-8407.04437.
  • 17. Barriere SL. Clinical, economic and societal impact of antibiotic resistance. Expert Opin Pharmacother. 2015;16(2):151-3. doi: 10.1517/14656566.2015.983077. [PubMed: 25483564].
  • 18. Cassell GH. Emergent antibiotic resistance: health risks and economic impact. FEMS Immunol Med Microbiol. 1997;18(4):271-4. doi: 10.1111/j.1574-695X.1997.tb01055.x. [PubMed: 9348162].
  • 19. Theuretzbacher U. Global antibacterial resistance: The never-ending story. J Glob Antimicrob Resist. 2013;1(2):63-9. doi: 10.1016/j.jgar.2013.03.010. [PubMed: 27873580].
  • 20. Saleem M. Natural Products as Antimicrobial Agents - an Update. 2014:219-94. doi: 10.1002/9783527676132.ch9.
  • 21. Sampedro J, Valdivia ER. New antimicrobial agents of plant origin. Antimicrobial compounds. Berlin Heidelberg: Springer; 2013. p. 83-114.
  • 22. Tirumalasetty J, Anuradha B, Praveena A. Antimicrobial activity of methanolic extracts of Azadirachta indica, Rosmarinus officinalis and Lagenaria siceraria leaves on some important pathogenic organisms. J Chem Pharm Res. 2014;6:766-70.
  • 23. Autade RH, Saini S, Reddy PG, Deorukhkar SC, Padmajakshi G. Effect of Neem extract against opportunistic bacterial and fungal pathogens associated with AIDS. Int J Curr Microbiol Appl Sci. 2015;4:988-99.
  • 24. Mamman PH, Mshelia WP, Susbatrus SC, Sambo KW. Antibacterial effects of crude extract of Azadirachta indica against Escherichia coli, salmonella spp and Staphylococcus aureus. Int J Med Sci. 2013;5(14-8).
  • 25. Rasool M, Malik A, Arooj M, Alam MZ, Alam Q, Awan M . Evaluation of antimicrobial activity of ethanolic extracts of Azadirachta indica and Psidium guajava against clinically important bacteria at varying pH and temperature. Biomed Res. 2017;28:134-9.
  • PDF ( 196.91 KB)
  • Supplementary Material : Download

Cited By:

Creative Commons License Except where otherwise noted, this work is licensed under Creative Commons Attribution Non Commercial 4.0 International License .

Search Relations:


  • Leila Maleki: [PubMed] [Scholar]
  • Tahereh Sadeghian-Rizi: [PubMed] [Scholar]
  • Mostafa Ghannadian: [PubMed] [Scholar]
  • Mohammad Hossein Sanati: [PubMed] [Scholar]
  • Shahin Shafizadegan: [PubMed] [Scholar]
  • Hojjat Sadeghi-Aliabadi: [PubMed] [Scholar]
  • Related Article in PubMed
  • Related Article in Google Scholar

Create Citiation Alert
via Google Reader

Readers' Comments