Avicenna Journal of Clinical Microbiology and Infection

Published by: Kowsar

Isolation and Identification of Bacillus Species From Soil and Evaluation of Their Antibacterial Properties

Mansour Amin 1 , Zeinab Rakhisi 2 , * and Amanollah Zarei Ahmady 3
Authors Information
1 Health Research Institute, Infectious and Tropical Diseases Research Center, Department of Microbiology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran
2 School of Basic Sciences, Islamic Azad University, Fars Science and Research Branch, Shiraz, IR Iran
3 Nanotechnology Research Center, Faculty of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran
Article information
  • Avicenna Journal of Clinical Microbiology and Infection: February 01, 2015, 2 (1); e23233
  • Published Online: February 20, 2015
  • Article Type: Research Article
  • Received: September 29, 2014
  • Revised: December 4, 2014
  • Accepted: December 17, 2014
  • DOI: 10.17795/ajcmi-23233

To Cite: Amin M, Rakhisi Z, Zarei Ahmady A. Isolation and Identification of Bacillus Species From Soil and Evaluation of Their Antibacterial Properties, Avicenna J Clin Microb Infec. 2015 ;2(1):e23233. doi: 10.17795/ajcmi-23233.

Copyright © 2015, Hamadan University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Materials and Methods
4. Results
5. Discussion
  • 1. Waites MJ, Morgan NL, Rockey JS, Higton G. Industrial Microbiology an Introduction. 2008;
  • 2. Kuta FA. Antifungal effects of Calotropis Procera stem bank extract against Trichoplyton gypseun and Epiderinoplyton Flocosum. Afr J Biotechnol. 2008; 7(13): 2116-8
  • 3. McKeen CD, Reilly CC, Pusey PL. Production and partial characterization of antifungal substances antagonistic to Monilinia fructicola from Bacillus subtilis. Phytopathology. 1986; 76(2): 136-9
  • 4. Silo-Suh LA, Lethbridge BJ, Raffel SJ, He H, Clardy J, Handelsman J. Biological activities of two fungistatic antibiotics produced by Bacillus cereus UW85. Appl Environ Microbiol. 1994; 60(6): 2023-30[PubMed]
  • 5. Leifert C, Li H, Chidburee S, Hampson S, Workman S, Sigee D, et al. Antibiotic production and biocontrol activity by Bacillus subtilis CL27 and Bacillus pumilus CL45. J Appl Bacteriol. 1995; 78(2): 97-108[PubMed]
  • 6. Priest FG. Biotechnology Hand Books, Bacillus. 1989; : 293-320
  • 7. Debabov VG. The Industrial Use of Bacilli. The Molecular Biology of the Bacilli. 1982;
  • 8. Violeta O, Oana S, Matilda C, Maria CD, Catalina V, Gheorghe C, et al. Production of biosurfactants and antifungal compounds by new strains of Bacillus spp. isolated from different sources. Rom Biotech Lett. 2011; 16(1): 84-91
  • 9. Mathur A, Rawat A, Bhatt G, Baweja S, Ahmad F, Grover A, et al. Isolation of Bacillus producing chitinase from soil: production and purification of chito-oligosaccharides from chitin extracted from fresh water crustaceans and antimicrobial activity of chitinase. Recent Res Sci Technol. 2011; 3(11): 1-6
  • 10. Ghribi D, Abdelkefi-Mesrati L, Mnif I, Kammoun R, Ayadi I, Saadaoui I, et al. Investigation of antimicrobial activity and statistical optimization of Bacillus subtilis SPB1 biosurfactant production in solid-state fermentation. J Biomed Biotechnol. 2012; 2012: 373682[DOI][PubMed]
  • 11. Issazadeh K, karimi-Rad S, Zarrabi S, Rahimibashar MR. Antagonism of Bacillus species against Xanthomonas campestris pv. campestris and Pectobacterium carotovorum subsp. carotovurum. Afr J Microbiol Res. 2012; 6(7): 1615-20
  • 12. Kumar SN, Siji JV, Ramya R, Nambisan B, Mohandas C. Improvement of antimicrobial activity of compounds produced by Bacillus sp. associated with a Rhabditid sp. (entomopathogenic nematode) by chancing carbon and nitrogen sources in fermentation media. J Microbiol Biotechnol Food Sci. 2012; 1: 1424-38
  • 13. Bacteria exhibiting antimicrobial activities; screening for antibiotics and the associated genetic studies. : 230-8
  • 14. Forbes BA, Sahm DF, Welssfeld AS. Baily and Scott's diagnostic microbiology. 2007;
  • 15. Amin M, Jorfi M, Khosravi AD, Samarbafzadeh AR, Sheikh AF. Isolation and identification of Lactobacillus casei and Lactobacillus plantarum from plants by PCR and detection of their antibacterial activity. J Biol Sci. 2009; 9(8): 810-4
  • 16. Berkeley RCW, Logan NA, Shute LA, Capey AG. Methods in Microbiology. 1984; : 292-323
  • 17. Claus D, Berkeley RCW. Bergey's manual of systematic bacteriology. 1986; : 1105-38
  • 18. Schmidt FR. The challenge of multidrug resistance: actual strategies in the development of novel antibacterials. Appl Microbiol Biotechnol. 2004; 63(4): 335-43[DOI][PubMed]
  • 19. Coates A, Hu Y, Bax R, Page C. The future challenges facing the development of new antimicrobial drugs. Nat Rev Drug Discov. 2002; 1(11): 895-910[DOI][PubMed]
  • 20. Jensen MJ, Wright DN. Chemotherapeutic agents. Microbiology for the health sciences. 1997;
  • 21. Prescott ML, Harley PJ, Klein AD. Microbiology. 2008;
  • 22. Oscariz JC, Lasa I, Pisabarro AG. Detection and characterization of cerein 7, a new bacteriocin produced by Bacillus cereus with a broad spectrum of activity. FEMS Microbiol Lett. 1999; 178(2): 337-41[PubMed]
  • 23. Aslim B, Saglam N, Beyatli Y. Determination of some properties of Bacillus isolated from soil. Turk J Biol. 2002; 26: 41-8
  • 24. Basurto-Cadena MG, Vazquez-Arista M, Garcia-Jimenez J, Salcedo-Hernandez R, Bideshi DK, Barboza-Corona JE. Isolation of a new Mexican strain of Bacillus subtilis with antifungal and antibacterial activities. Scientific World Journal. 2012; 2012: 384978[DOI][PubMed]

Cited By:

Creative Commons License Except where otherwise noted, this work is licensed under Creative Commons Attribution Non Commercial 4.0 International License .

Search Relations:


  • Mansour Amin: [PubMed] [Scholar]
  • Zeinab Rakhisi: [PubMed] [Scholar]
  • Amanollah Zarei Ahmady: [PubMed] [Scholar]
  • Related Article in PubMed
  • Related Article in Google Scholar

Create Citiation Alert
via Google Reader

Readers' Comments