Avicenna Journal of Clinical Microbiology and Infection

Published by: Kowsar

Purification, Extraction and Visualization of Lipopolysaccharide of Escherichia coli From Urine Samples of Patients With Urinary Tract Infection

Fariha Akhter Chowdhury 1 , Mohammad Nurul Islam 1 , Anamika Saha 1 , Sabrina Mahboob 1 , Abu Syed Md. Mosaddek 2 , Md Omar Faruque 3 , Most. Fahmida Begum 4 and Rajib Bhattacharjee 1 , *
Authors Information
1 Department of Pharmaceutical Sciences, North South University, Dhaka, Bangladesh
2 Department of Pharmacology, Uttara Adhunik Medical College, Dhaka, Bangladesh
3 Department of Physiology and Molecular Biology, Bangladesh University of Health Sciences, Dhaka, Bangladesh
4 Department of Microbiology, Uttara Adhunik Medical College, Dhaka, Bangladesh
Article information
  • Avicenna Journal of Clinical Microbiology and Infection: November 01, 2015, 2 (4); e31990
  • Published Online: November 23, 2015
  • Article Type: Research Article
  • Received: August 9, 2015
  • Revised: August 22, 2015
  • Accepted: September 19, 2015
  • DOI: 10.17795/ajcmi-31990

To Cite: Chowdhury F A, Nurul Islam M, Saha A, Mahboob S, Syed Md. Mosaddek A, et al. Purification, Extraction and Visualization of Lipopolysaccharide of Escherichia coli From Urine Samples of Patients With Urinary Tract Infection, Avicenna J Clin Microb Infec. 2015 ;2(4):e31990. doi: 10.17795/ajcmi-31990.

Copyright © 2015, Hamadan University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Patients and Methods
4. Results
5. Discussion
  • 1. Bashir MF, Qazi JI, Ahmad N, Riaz S. Diversity of urinary tract pathogens and drug resistant isolates of Escherichia coli in different age and gender groups of Pakistanis. Trop j pharm res. 2008; 7(3): 1025-31[DOI]
  • 2. Behzadi P, Behzadi E. The microbial agents of urinary tract infections at Central Laboratory of Dr. Shariati Hospital, Tehran, Iran. Turk Klin Tip Bilim. 2008; 28(4): 445
  • 3. Elgavish A. Effects of Escherichia coli and E. coli lipopolysaccharides on the function of human ureteral epithelial cells cultured in serum-free medium. Infect Immun. 1993; 61(8): 3304-12[PubMed]
  • 4. Erridge C, Bennett-Guerrero E, Poxton IR. Structure and function of lipopolysaccharides. Microbes Infect. 2002; 4(8): 837-51[PubMed]
  • 5. Rudick CN, Billips BK, Pavlov VI, Yaggie RE, Schaeffer AJ, Klumpp DJ. Host-pathogen interactions mediating pain of urinary tract infection. J Infect Dis. 2010; 201(8): 1240-9[DOI][PubMed]
  • 6. Hitchcock PJ, Brown TM. Morphological heterogeneity among Salmonella lipopolysaccharide chemotypes in silver-stained polyacrylamide gels. J Bacteriol. 1983; 154(1): 269-77[PubMed]
  • 7. Nerurkar A, Solanky P, Naik SS. Bacterial pathogen in urinary tract infection and antimicrobial susceptibility pattern. J Pharm Biomed Sci. 2012; 21: 1-3
  • 8. Apu AS, Chowdhury FA, Khatun F, Jamaluddin ATM, Pathan AH, Pal A. Phytochemical screening and in vitro evaluation of pharmacological activities of Aphanamixis polystachya (Wall) Parker fruit extracts. Trop J Pharm Res. 2013; 12(1): 111-6[DOI]
  • 9. Clinical and Laboratory Standards Institute (CLSI). . Performance standards for antimicrobial disk susceptibility tests: approved standard-CLSI document M02-A11. 11; 2012.
  • 10. Tsai CM, Frasch CE. A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels. Anal biochem. 1982; 119(1): 115-9
  • 11. Coomassie Blue Staining . 2009;
  • 12. Komala M, Kumar KPS. Urinary Tract Infection: Causes, Symptoms, Diagnosis and It’s Management. Ind J Res Pharma Biotech. 2013; 1(2): 226-33
  • 13. Farrell DJ, Morrissey I, De Rubeis D, Robbins M, Felmingham D. A UK multicentre study of the antimicrobial susceptibility of bacterial pathogens causing urinary tract infection. J Infect Dis. 2003; 46(2): 94-100
  • 14. Shahina Z, Islam MJ, Abedin J, Chowdhury AHMI, Arifuzzaman MD. A Study of Antibacterial Susceptibility and Resistance Pattern of E. coli Causing Urinary Tract Infection in Chittagong, Bangladesh. Asian J Biol Sci. 2011; 4: 548-55[DOI]
  • 15. Saeed MA, Haque A, Ali A, Mohsin M, Bashir S, Tariq A, et al. A profile of drug resistance genes and integrons in E. coli causing surgical wound infections in the Faisalabad region of Pakistan. J Antibiot (Tokyo). 2009; 62(6): 319-23[DOI][PubMed]
  • 16. Davis Jr MR, Goldberg JB. Purification and visualization of lipopolysaccharide from Gram-negative bacteria by hot aqueous-phenol extraction. J Vis Exp. 2012; (63)[DOI][PubMed]
  • 17. Hitchcock PJ. Aberrant migration of lipopolysaccharide in sodium dodecyl sulfate/polyacrylamide gel electrophoresis. Eur J Biochem. 1983; 133(3): 685-8[PubMed]
  • 18. Wang X, Zhang C, Shi F, Hu X. Endotoxins: Structure, Function and Recognition. Subcellular Biochemistry. 2010; : 27-51
  • 19. Wu MC, Heath EC. Isolation and characterization of lipopolysaccharide protein from Escherichia coli. Proceedings of the National Academy of Sciences. 1973; 70(9): 2572-6
  • 20. Weiss W, Weiland F, Görg A. Proteomics. Methods in Molecular Biology™. 2009; : 59-82
  • 21. Rezania S, Amirmozaffari N, Tabarraei B, Jeddi-Tehrani M, Zarei O, Alizadeh R, et al. Extraction, Purification and Characterization of Lipopolysaccharide from Escherichia coli and Salmonella typhi. Avicenna J Med Biotechnol. 2011; 3(1): 3-9[PubMed]
  • 22. Hendrick CA, Sequeira L. Lipopolysaccharide-Defective Mutants of the Wilt Pathogen Pseudomonas solanacearum. Appl Environ Microbiol. 1984; 48(1): 94-101[PubMed]
  • 23. Bantroch S, Buhler T, Lam JS. Appropriate coating methods and other conditions for enzyme-linked immunosorbent assay of smooth, rough, and neutral lipopolysaccharides of Pseudomonas aeruginosa. Clin Diagn Lab Immunol. 1994; 1(1): 55-62[PubMed]
  • 24. Gomaa AA, Klumpe HE, Luo ML, Selle K, Barrangou R, Beisel CL. Programmable removal of bacterial strains by use of genome-targeting CRISPR-Cas systems. MBio. 2014; 5(1)-13[DOI][PubMed]
  • 25. Cray WC, Moon HW. Experimental infection of calves and adult cattle with Escherichia coli O157:H7. Appl Environ Microbiol. 1995; 61(4): 1586-90[PubMed]
  • 26. Tenaillon O, Skurnik D, Picard B, Denamur E. The population genetics of commensal Escherichia coli. Nat Rev Microbiol. 2010; 8(3): 207-17[DOI][PubMed]
Creative Commons License Except where otherwise noted, this work is licensed under Creative Commons Attribution Non Commercial 4.0 International License .

Search Relations:



Create Citiation Alert
via Google Reader

Readers' Comments