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Prevalence of blaPER-1 in ESBL-producing Clinical Isolates of Pseudomonas aeruginosa in the ICUs of Several Iranian Hospitals


1 Microbiology Department, Faculty of Medicine, AJA University of Medical Sciences, Tehran, IR Iran
2 Department of Microbiology, Fasa University of Medical Sciences, Fasa, IR Iran
3 Medical Nanotechnology, Baqiyatallah University of Medical Sciences, Nano Biotechnology Research Center, Tehran, IR Iran
*Corresponding author: Danial Ashiani, Medical Nanotechnology, Baqiyatallah University of Medical Sciences, Nano Biotechnology Research Center, Tehran, IR Iran, E-mail: lab.microbiology49@gmail.com.
Avicenna Journal of Clinical Microbiology and Infection. 3(4): e39071 , DOI: 10.17795/ajcmi-39071
Article Type: Letter; Received: May 10, 2016; Revised: Aug 1, 2016; Accepted: Aug 3, 2016; epub: Aug 14, 2016; collection: Nov 2016

Keywords: blaPER-1; P.aeruginosa; ESBL Positive; Iranian Hospitals


Dear Editor,


Pseudomonas aeruginosa strains are resistant to most first- and second-generation cephalosporins, but these isolates are susceptible to third-generation cephalosporins, such as cefsulodin and ceftazidime, as nontoxic alternatives to aminoglycosides. However, resistance to beta-lactams occurs via plasmid or chromosomal beta-lactamases, which hydrolyze their active ring (1, 2). PER-1 is a class A enzyme that is related to a high level of resistance to cephems, monobactams, and ceftazidime (3, 4). Several previous studies have demonstrated that blaPER-1 is widespread in Turkey and has been detected in nearly 50% of extended-spectrum beta-lactamase (ESBL)-positive burn isolates in Iran (5). Previous studies have demonstrated that PER-1-positive isolates had high levels of resistance to penicillin, ceftazidime, and ceftriaxone antibiotics (6, 7). The aim of this study was to detect the prevalence of the blaPER-1 gene in several hospitals in the Iranian cities of Tehran, Shiraz, Kermanshah, Ilam, Kerman, and Ahvaz.


A total of 85 clinical isolates of P. aeruginosa were collected from patients in the intensive care units (ICUs) of hospitals in Tehran (Motahari and Pediatrics Center), Shiraz (Namazi), Kermanshah (Imam), Ilam (Imam Khomeini), Kerman (Ba Honar), and Ahvaz (Taleghani and Golestan). Antibiotic susceptibility testing was conducted based on the Kirby-Bauer method and according to the Clinical and Laboratory Standards Institute (CLSI 2012) guidelines. P. aeruginosa ATCC27853 was used as the control strain. SPSS version 16 and Excel software were used in this study. Student’s t-test was used for the analysis of results. P values of < 0.05 (CI = 0.95%) were considered to have statistical significance.


The majority of the isolates came from Tehran and Shiraz, and most were from urine and lung infections. The frequency of isolates among men and women was not significantly different.


While imipenem and meropenem were the most effective drugs, resistance to cefpodoxime was 94.1%. The comparison of the antibiotic susceptibility profiles of ESBL-positive and -negative isolates is shown in Table 1. The ESBL-positive isolates were resistant to the majority of antibiotics tested, especially third-generation cephalosporins. The majority of urine isolates were resistant to all antibiotics, mainly due to the kidney route of drug elimination. The resistance rates to ceftazidime and ceftriaxone were 89% and 88%, respectively. Thirty (35.3%) isolates were ESBL-positive P. aeruginosa. The drug resistance of the ESBL-producing isolates to the tested antibiotics was higher than that of the ESBL-negative isolates. The majority of ESBL-positive isolates of P. aeruginosa were from urine samples.


Table 1.
Differences in Antibiotic Resistance Between ESBL-positive and ESBL-negative Isolates P. aeruginosa

The prevalence of blaPER-1 was 30% (n = 9) in the ESBL-positive isolates. Among these nine isolates, four, three, and two blaPER-1-positive isolates were from Tehran, Shiraz and Ahvaz, respectively.


The ESBL-positive isolates had a higher rate of drug resistance. The resistance rates to ceftazidime and ceftriaxone were 63% and 64%, respectively. Urine isolates harbored a significantly higher rate of the blaPER-1 gene among the different clinical sites. Furthermore, the isolates with resistance to all of the third-generation cephalosporins harbored a higher prevalence of the blaPER-1 beta-lactamase. Data on the prevalence of the blaPER-1 gene in the Middle East are limited. In Japoni’s survey, in Shiraz, ESBLs were detected in only three (4.3%) isolates (8). In a previous study, the prevalence of blaVEB-1 was 40% in these isolates (9).


Approximately one-third of the isolates in the present study were ESBL-positive. The cities of Tehran, Shiraz, and Ahwaz demonstrated more ESBL isolates of P. aeruginosa, and the isolates from these cities amplified blaPER-1. Moreover, nearly one-third of the ESBL producers amplified the blaPER-1 gene.

Acknowledgments

This study was supported by Bandar Abbas University of Medical Sciences.

Footnotes

Authors’ Contribution: Abdolmajid Ghasemian and Farshad Nojoomi designed and guided the study, with the help of other authors.
Conflicts of Interest: There are no conflicts of interest to declare.

References


  • 1. Basavraj N, Suryawanshi NM. Antimicrobial Resistance in P. aeruginosa-A Review. J Med Educat Res. 2012;2
  • 2. Fazeli H, Sadighian H, Nasr-Esfahani B, Pourmand MR. Identification of class-1 integron and various β-lactamase classes among clinical isolates of Pseudomonas aeruginosa at children's medical center hospital. J Med Bacteriol. 2015;1(3-4):25-36.
  • 3. Endimiani A, Luzzaro F, Pini B, Amicosante G, Rossolini GM, Toniolo AQ. Pseudomonas aeruginosa bloodstream infections: risk factors and treatment outcome related to expression of the PER-1 extended-spectrum beta-lactamase. BMC Infect Dis. 2006;6:52. [DOI] [PubMed]
  • 4. Strateva T, Yordanov D. Pseudomonas aeruginosa - a phenomenon of bacterial resistance. J Med Microbiol. 2009;58(Pt 9):1133-48. [DOI] [PubMed]
  • 5. Mirsalehian A, Feizabadi M, Nakhjavani FA, Jabalameli F, Goli H, Kalantari N. Detection of VEB-1, OXA-10 and PER-1 genotypes in extended-spectrum beta-lactamase-producing Pseudomonas aeruginosa strains isolated from burn patients. Burns. 2010;36(1):70-4. [DOI] [PubMed]
  • 6. Shahcheraghi F, Nikbin VS, Feizabadi MM. Prevalence of ESBLs genes among multidrug-resistant isolates of Pseudomonas aeruginosa isolated from patients in Tehran. Microb Drug Resist. 2009;15(1):37-9. [DOI] [PubMed]
  • 7. Shacheraghi F, Shakibaie MR, Noveiri H. Molecular identification of ESBL Genes blaGES-blaVEB-blaCTX-M blaOXA-blaOXA-4, blaOXA-10 andblaPER-in Pseudomonas aeruginosa strains isolated from burn patients by PCR, RFLP and sequencing techniques. Int J Biol life Sci. 2010;3(6):138-42..
  • 8. Japoni A, Alborzi A, Kalani M, Nasiri J, Hayati M, Farshad S. Susceptibility patterns and cross-resistance of antibiotics against Pseudomonas aeruginosa isolated from burn patients in the South of Iran. Burns. 2006;32(3):343-7. [DOI] [PubMed]
  • 9. Davodian E, Sadeghifard N, Ghasemian A, Noorbakhsh S. Molecular detection of bla VEB-1 beta-lactamase encoding gene among extended spectrum B-Lactamase positive wound isolates of Pseudomonas aeruginosa. Arch Pediatr Infect Dis. 2015;3(4)

Table 1.

Differences in Antibiotic Resistance Between ESBL-positive and ESBL-negative Isolates P. aeruginosa

Disks/Isolates ESBL-Positive (%), n = 30 ESBL-Negative (%), n = 55
CPM 96 53
CAZ 89 63
IMI 22 17
MEM 23 18
CIP 89 65
OFX 78 44
TN 82 42
GEN 68 46
Abbreviations: CAZ, ceftazidime; CIP, ciprofloxacin; CPM, cefepime; GEN, gentamicin; IMI, imipenem; MEM, meropenem; OFX, ofloxacin; TN, tobramycin.